Molecular Studies on Infectious Bronchitis Virus Isolated in Poland
نویسندگان
چکیده
The comparison of the conventional (aimed at the UTR3 fragment) and nested RT-PCR (aimed at the N gene fragment) based methods revealed that nested PCR was about 1000 times more sensitive for the detection of infectious bronchitis virus (IBV). Molecular differentiation of 793/B, D274, and Mass IBV types was also achieved in multiplex nested PCR. Eight from nine studied Polish isolates had the characteristic band for 793/B and five simultaneously to D274 type. No band was obtained in the case of 162/97 isolate. The phylogenetic analysis of nucleotide sequences of IBV isolates from 1997-2006 revealed that 793/B and QX types are present in Poland. Two isolates have an unclear relationship with the rest of the strains and formed separate branches of the phylogenetic tree.
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